Molecular cloning and characterization of two genes encoding dihydroflavonol-4-reductase from Populus trichocarpa.
Identifieur interne : 002987 ( Main/Exploration ); précédent : 002986; suivant : 002988Molecular cloning and characterization of two genes encoding dihydroflavonol-4-reductase from Populus trichocarpa.
Auteurs : Yan Huang [République populaire de Chine] ; Jiqing Gou ; Zhichun Jia ; Li Yang ; Yimin Sun ; Xunyan Xiao ; Feng Song ; Keming LuoSource :
- PloS one [ 1932-6203 ] ; 2012.
Descripteurs français
- KwdFr :
- ARN messager (génétique), ARN messager (métabolisme), Alcohol oxidoreductases (composition chimique), Alcohol oxidoreductases (génétique), Alcohol oxidoreductases (métabolisme), Anthocyanes (métabolisme), Clonage moléculaire (MeSH), Données de séquences moléculaires (MeSH), Fleurs (génétique), Gènes de plante (génétique), Phylogenèse (MeSH), Pigmentation (génétique), Populus (enzymologie), Populus (génétique), Proanthocyanidines (métabolisme), Réaction de polymérisation en chaine en temps réel (MeSH), Régulation de l'expression des gènes codant pour des enzymes (MeSH), Régulation de l'expression des gènes végétaux (MeSH), Séquence d'acides aminés (MeSH), Séquence nucléotidique (MeSH), Tabac (génétique), Végétaux génétiquement modifiés (MeSH).
- MESH :
- composition chimique : Alcohol oxidoreductases.
- enzymologie : Populus.
- génétique : ARN messager, Alcohol oxidoreductases, Fleurs, Gènes de plante, Pigmentation, Populus, Tabac.
- métabolisme : ARN messager, Alcohol oxidoreductases, Anthocyanes, Proanthocyanidines.
- Clonage moléculaire, Données de séquences moléculaires, Phylogenèse, Réaction de polymérisation en chaine en temps réel, Régulation de l'expression des gènes codant pour des enzymes, Régulation de l'expression des gènes végétaux, Séquence d'acides aminés, Séquence nucléotidique, Végétaux génétiquement modifiés.
English descriptors
- KwdEn :
- Alcohol Oxidoreductases (chemistry), Alcohol Oxidoreductases (genetics), Alcohol Oxidoreductases (metabolism), Amino Acid Sequence (MeSH), Anthocyanins (metabolism), Base Sequence (MeSH), Cloning, Molecular (MeSH), Flowers (genetics), Gene Expression Regulation, Enzymologic (MeSH), Gene Expression Regulation, Plant (MeSH), Genes, Plant (genetics), Molecular Sequence Data (MeSH), Phylogeny (MeSH), Pigmentation (genetics), Plants, Genetically Modified (MeSH), Populus (enzymology), Populus (genetics), Proanthocyanidins (metabolism), RNA, Messenger (genetics), RNA, Messenger (metabolism), Real-Time Polymerase Chain Reaction (MeSH), Tobacco (genetics).
- MESH :
- chemical , chemistry : Alcohol Oxidoreductases.
- chemical , genetics : Alcohol Oxidoreductases, RNA, Messenger.
- chemical , metabolism : Alcohol Oxidoreductases, Anthocyanins, Proanthocyanidins, RNA, Messenger.
- enzymology : Populus.
- genetics : Flowers, Genes, Plant, Pigmentation, Populus, Tobacco.
- Amino Acid Sequence, Base Sequence, Cloning, Molecular, Gene Expression Regulation, Enzymologic, Gene Expression Regulation, Plant, Molecular Sequence Data, Phylogeny, Plants, Genetically Modified, Real-Time Polymerase Chain Reaction.
Abstract
Dihydroflavonol 4-reductase (DFR, EC 1.1.1.219) is a rate-limited enzyme in the biosynthesis of anthocyanins and condensed tannins (proanthocyanidins) that catalyzes the reduction of dihydroflavonols to leucoanthocyanins. In this study, two full-length transcripts encoding for PtrDFR1 and PtrDFR2 were isolated from Populus trichocarpa. Sequence alignment of the two PtrDFRs with other known DFRs reveals the homology of these genes. The expression profile of PtrDFRs was investigated in various tissues of P. trichocarpa. To determine their functions, two PtrDFRs were overexpressed in tobacco (Nicotiana tabacum) via Agrobacterium-mediated transformation. The associated color change in the flowers was observed in all 35S:PtrDFR1 lines, but not in 35S:PtrDFR2 lines. Compared to the wild-type control, a significantly higher accumulation of anthocyanins was detected in transgenic plants harboring the PtrDFR1. Furthermore, overexpressing PtrDFR1 in Chinese white poplar (P. tomentosa Carr.) resulted in a higher accumulation of both anthocyanins and condensed tannins, whereas constitutively expressing PtrDFR2 only improved condensed tannin accumulation, indicating the potential regulation of condensed tannins by PtrDFR2 in the biosynthetic pathway in poplars.
DOI: 10.1371/journal.pone.0030364
PubMed: 22363429
PubMed Central: PMC3281835
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Molecular cloning and characterization of two genes encoding dihydroflavonol-4-reductase from Populus trichocarpa.</title><author><name sortKey="Huang, Yan" sort="Huang, Yan" uniqKey="Huang Y" first="Yan" last="Huang">Yan Huang</name><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Eco-Environments of Three Gorges Reservoir Region, Ministry of Education, Institute of Resources Botany, School of Life Sciences, Southwest University, Chongqing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Eco-Environments of Three Gorges Reservoir Region, Ministry of Education, Institute of Resources Botany, School of Life Sciences, Southwest University, Chongqing</wicri:regionArea><wicri:noRegion>Chongqing</wicri:noRegion></affiliation></author><author><name sortKey="Gou, Jiqing" sort="Gou, Jiqing" uniqKey="Gou J" first="Jiqing" last="Gou">Jiqing Gou</name></author><author><name sortKey="Jia, Zhichun" sort="Jia, Zhichun" uniqKey="Jia Z" first="Zhichun" last="Jia">Zhichun Jia</name></author><author><name sortKey="Yang, Li" sort="Yang, Li" uniqKey="Yang L" first="Li" last="Yang">Li Yang</name></author><author><name sortKey="Sun, Yimin" sort="Sun, Yimin" uniqKey="Sun Y" first="Yimin" last="Sun">Yimin Sun</name></author><author><name sortKey="Xiao, Xunyan" sort="Xiao, Xunyan" uniqKey="Xiao X" first="Xunyan" last="Xiao">Xunyan Xiao</name></author><author><name sortKey="Song, Feng" sort="Song, Feng" uniqKey="Song F" first="Feng" last="Song">Feng Song</name></author><author><name sortKey="Luo, Keming" sort="Luo, Keming" uniqKey="Luo K" first="Keming" last="Luo">Keming Luo</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2012">2012</date><idno type="RBID">pubmed:22363429</idno><idno type="pmid">22363429</idno><idno type="doi">10.1371/journal.pone.0030364</idno><idno type="pmc">PMC3281835</idno><idno type="wicri:Area/Main/Corpus">002B26</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">002B26</idno><idno type="wicri:Area/Main/Curation">002B26</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">002B26</idno><idno type="wicri:Area/Main/Exploration">002B26</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Molecular cloning and characterization of two genes encoding dihydroflavonol-4-reductase from Populus trichocarpa.</title><author><name sortKey="Huang, Yan" sort="Huang, Yan" uniqKey="Huang Y" first="Yan" last="Huang">Yan Huang</name><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Eco-Environments of Three Gorges Reservoir Region, Ministry of Education, Institute of Resources Botany, School of Life Sciences, Southwest University, Chongqing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Eco-Environments of Three Gorges Reservoir Region, Ministry of Education, Institute of Resources Botany, School of Life Sciences, Southwest University, Chongqing</wicri:regionArea><wicri:noRegion>Chongqing</wicri:noRegion></affiliation></author><author><name sortKey="Gou, Jiqing" sort="Gou, Jiqing" uniqKey="Gou J" first="Jiqing" last="Gou">Jiqing Gou</name></author><author><name sortKey="Jia, Zhichun" sort="Jia, Zhichun" uniqKey="Jia Z" first="Zhichun" last="Jia">Zhichun Jia</name></author><author><name sortKey="Yang, Li" sort="Yang, Li" uniqKey="Yang L" first="Li" last="Yang">Li Yang</name></author><author><name sortKey="Sun, Yimin" sort="Sun, Yimin" uniqKey="Sun Y" first="Yimin" last="Sun">Yimin Sun</name></author><author><name sortKey="Xiao, Xunyan" sort="Xiao, Xunyan" uniqKey="Xiao X" first="Xunyan" last="Xiao">Xunyan Xiao</name></author><author><name sortKey="Song, Feng" sort="Song, Feng" uniqKey="Song F" first="Feng" last="Song">Feng Song</name></author><author><name sortKey="Luo, Keming" sort="Luo, Keming" uniqKey="Luo K" first="Keming" last="Luo">Keming Luo</name></author></analytic><series><title level="j">PloS one</title><idno type="eISSN">1932-6203</idno><imprint><date when="2012" type="published">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Alcohol Oxidoreductases (chemistry)</term><term>Alcohol Oxidoreductases (genetics)</term><term>Alcohol Oxidoreductases (metabolism)</term><term>Amino Acid Sequence (MeSH)</term><term>Anthocyanins (metabolism)</term><term>Base Sequence (MeSH)</term><term>Cloning, Molecular (MeSH)</term><term>Flowers (genetics)</term><term>Gene Expression Regulation, Enzymologic (MeSH)</term><term>Gene Expression Regulation, Plant (MeSH)</term><term>Genes, Plant (genetics)</term><term>Molecular Sequence Data (MeSH)</term><term>Phylogeny (MeSH)</term><term>Pigmentation (genetics)</term><term>Plants, Genetically Modified (MeSH)</term><term>Populus (enzymology)</term><term>Populus (genetics)</term><term>Proanthocyanidins (metabolism)</term><term>RNA, Messenger (genetics)</term><term>RNA, Messenger (metabolism)</term><term>Real-Time Polymerase Chain Reaction (MeSH)</term><term>Tobacco (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN messager (génétique)</term><term>ARN messager (métabolisme)</term><term>Alcohol oxidoreductases (composition chimique)</term><term>Alcohol oxidoreductases (génétique)</term><term>Alcohol oxidoreductases (métabolisme)</term><term>Anthocyanes (métabolisme)</term><term>Clonage moléculaire (MeSH)</term><term>Données de séquences moléculaires (MeSH)</term><term>Fleurs (génétique)</term><term>Gènes de plante (génétique)</term><term>Phylogenèse (MeSH)</term><term>Pigmentation (génétique)</term><term>Populus (enzymologie)</term><term>Populus (génétique)</term><term>Proanthocyanidines (métabolisme)</term><term>Réaction de polymérisation en chaine en temps réel (MeSH)</term><term>Régulation de l'expression des gènes codant pour des enzymes (MeSH)</term><term>Régulation de l'expression des gènes végétaux (MeSH)</term><term>Séquence d'acides aminés (MeSH)</term><term>Séquence nucléotidique (MeSH)</term><term>Tabac (génétique)</term><term>Végétaux génétiquement modifiés (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Alcohol Oxidoreductases</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Alcohol Oxidoreductases</term><term>RNA, Messenger</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Alcohol Oxidoreductases</term><term>Anthocyanins</term><term>Proanthocyanidins</term><term>RNA, Messenger</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Alcohol oxidoreductases</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Flowers</term><term>Genes, Plant</term><term>Pigmentation</term><term>Populus</term><term>Tobacco</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN messager</term><term>Alcohol oxidoreductases</term><term>Fleurs</term><term>Gènes de plante</term><term>Pigmentation</term><term>Populus</term><term>Tabac</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>ARN messager</term><term>Alcohol oxidoreductases</term><term>Anthocyanes</term><term>Proanthocyanidines</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Base Sequence</term><term>Cloning, Molecular</term><term>Gene Expression Regulation, Enzymologic</term><term>Gene Expression Regulation, Plant</term><term>Molecular Sequence Data</term><term>Phylogeny</term><term>Plants, Genetically Modified</term><term>Real-Time Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Clonage moléculaire</term><term>Données de séquences moléculaires</term><term>Phylogenèse</term><term>Réaction de polymérisation en chaine en temps réel</term><term>Régulation de l'expression des gènes codant pour des enzymes</term><term>Régulation de l'expression des gènes végétaux</term><term>Séquence d'acides aminés</term><term>Séquence nucléotidique</term><term>Végétaux génétiquement modifiés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Dihydroflavonol 4-reductase (DFR, EC 1.1.1.219) is a rate-limited enzyme in the biosynthesis of anthocyanins and condensed tannins (proanthocyanidins) that catalyzes the reduction of dihydroflavonols to leucoanthocyanins. In this study, two full-length transcripts encoding for PtrDFR1 and PtrDFR2 were isolated from Populus trichocarpa. Sequence alignment of the two PtrDFRs with other known DFRs reveals the homology of these genes. The expression profile of PtrDFRs was investigated in various tissues of P. trichocarpa. To determine their functions, two PtrDFRs were overexpressed in tobacco (Nicotiana tabacum) via Agrobacterium-mediated transformation. The associated color change in the flowers was observed in all 35S:PtrDFR1 lines, but not in 35S:PtrDFR2 lines. Compared to the wild-type control, a significantly higher accumulation of anthocyanins was detected in transgenic plants harboring the PtrDFR1. Furthermore, overexpressing PtrDFR1 in Chinese white poplar (P. tomentosa Carr.) resulted in a higher accumulation of both anthocyanins and condensed tannins, whereas constitutively expressing PtrDFR2 only improved condensed tannin accumulation, indicating the potential regulation of condensed tannins by PtrDFR2 in the biosynthetic pathway in poplars.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">22363429</PMID><DateCompleted><Year>2012</Year><Month>06</Month><Day>29</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1932-6203</ISSN><JournalIssue CitedMedium="Internet"><Volume>7</Volume><Issue>2</Issue><PubDate><Year>2012</Year></PubDate></JournalIssue><Title>PloS one</Title><ISOAbbreviation>PLoS One</ISOAbbreviation></Journal><ArticleTitle>Molecular cloning and characterization of two genes encoding dihydroflavonol-4-reductase from Populus trichocarpa.</ArticleTitle><Pagination><MedlinePgn>e30364</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1371/journal.pone.0030364</ELocationID><Abstract><AbstractText>Dihydroflavonol 4-reductase (DFR, EC 1.1.1.219) is a rate-limited enzyme in the biosynthesis of anthocyanins and condensed tannins (proanthocyanidins) that catalyzes the reduction of dihydroflavonols to leucoanthocyanins. In this study, two full-length transcripts encoding for PtrDFR1 and PtrDFR2 were isolated from Populus trichocarpa. Sequence alignment of the two PtrDFRs with other known DFRs reveals the homology of these genes. The expression profile of PtrDFRs was investigated in various tissues of P. trichocarpa. To determine their functions, two PtrDFRs were overexpressed in tobacco (Nicotiana tabacum) via Agrobacterium-mediated transformation. The associated color change in the flowers was observed in all 35S:PtrDFR1 lines, but not in 35S:PtrDFR2 lines. Compared to the wild-type control, a significantly higher accumulation of anthocyanins was detected in transgenic plants harboring the PtrDFR1. Furthermore, overexpressing PtrDFR1 in Chinese white poplar (P. tomentosa Carr.) resulted in a higher accumulation of both anthocyanins and condensed tannins, whereas constitutively expressing PtrDFR2 only improved condensed tannin accumulation, indicating the potential regulation of condensed tannins by PtrDFR2 in the biosynthetic pathway in poplars.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Huang</LastName><ForeName>Yan</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Key Laboratory of Eco-Environments of Three Gorges Reservoir Region, Ministry of Education, Institute of Resources Botany, School of Life Sciences, Southwest University, Chongqing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gou</LastName><ForeName>Jiqing</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Jia</LastName><ForeName>Zhichun</ForeName><Initials>Z</Initials></Author><Author ValidYN="Y"><LastName>Yang</LastName><ForeName>Li</ForeName><Initials>L</Initials></Author><Author ValidYN="Y"><LastName>Sun</LastName><ForeName>Yimin</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Xiao</LastName><ForeName>Xunyan</ForeName><Initials>X</Initials></Author><Author ValidYN="Y"><LastName>Song</LastName><ForeName>Feng</ForeName><Initials>F</Initials></Author><Author ValidYN="Y"><LastName>Luo</LastName><ForeName>Keming</ForeName><Initials>K</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2012</Year><Month>02</Month><Day>17</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>PLoS One</MedlineTA><NlmUniqueID>101285081</NlmUniqueID><ISSNLinking>1932-6203</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000872">Anthocyanins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D044945">Proanthocyanidins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012333">RNA, Messenger</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.1.-</RegistryNumber><NameOfSubstance UI="D000429">Alcohol Oxidoreductases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.1.1.-</RegistryNumber><NameOfSubstance UI="C056570">dihydroflavanol 4-reductase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000429" MajorTopicYN="N">Alcohol Oxidoreductases</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000872" MajorTopicYN="N">Anthocyanins</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001483" MajorTopicYN="N">Base Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D003001" MajorTopicYN="N">Cloning, Molecular</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D035264" MajorTopicYN="N">Flowers</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015971" MajorTopicYN="N">Gene Expression Regulation, Enzymologic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017343" MajorTopicYN="N">Genes, Plant</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010802" MajorTopicYN="N">Phylogeny</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010858" MajorTopicYN="N">Pigmentation</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D044945" MajorTopicYN="N">Proanthocyanidins</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012333" MajorTopicYN="N">RNA, Messenger</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D060888" MajorTopicYN="N">Real-Time Polymerase Chain Reaction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014026" MajorTopicYN="N">Tobacco</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2011</Year><Month>07</Month><Day>14</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2011</Year><Month>12</Month><Day>15</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2012</Year><Month>2</Month><Day>25</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2012</Year><Month>3</Month><Day>1</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2012</Year><Month>6</Month><Day>30</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">22363429</ArticleId><ArticleId IdType="doi">10.1371/journal.pone.0030364</ArticleId><ArticleId IdType="pii">PONE-D-11-13397</ArticleId><ArticleId IdType="pmc">PMC3281835</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>New Phytol. 2006;172(1):47-62</Citation><ArticleIdList><ArticleId IdType="pubmed">16945088</ArticleId></ArticleIdList></Reference><Reference><Citation>Biotechnol Bioeng. 1990 Dec 5;36(10):993-1001</Citation><ArticleIdList><ArticleId IdType="pubmed">18595037</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 1993 Sep 1;90(17):8043-7</Citation><ArticleIdList><ArticleId IdType="pubmed">8367460</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Mol Biol. 1989 Nov;13(5):491-502</Citation><ArticleIdList><ArticleId IdType="pubmed">2491667</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 1998 Mar;116(3):1133-44</Citation><ArticleIdList><ArticleId IdType="pubmed">9501146</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant J. 1997 Mar;11(3):429-41</Citation><ArticleIdList><ArticleId IdType="pubmed">9107033</ArticleId></ArticleIdList></Reference><Reference><Citation>Trends Plant Sci. 1999 Oct;4(10):394-400</Citation><ArticleIdList><ArticleId IdType="pubmed">10498963</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Sci. 2001 Feb 5;160(3):463-472</Citation><ArticleIdList><ArticleId IdType="pubmed">11166433</ArticleId></ArticleIdList></Reference><Reference><Citation>Tree Physiol. 2010 Dec;30(12):1599-605</Citation><ArticleIdList><ArticleId IdType="pubmed">21084346</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Gen Genet. 1991 Nov;230(1-2):49-59</Citation><ArticleIdList><ArticleId IdType="pubmed">1720864</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Sci. 2000 Dec 7;160(1):49-56</Citation><ArticleIdList><ArticleId IdType="pubmed">11164576</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 2004 Mar;134(3):979-94</Citation><ArticleIdList><ArticleId IdType="pubmed">14976232</ArticleId></ArticleIdList></Reference><Reference><Citation>Arch Biochem Biophys. 2003 Apr 15;412(2):223-30</Citation><ArticleIdList><ArticleId IdType="pubmed">12667486</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 1984 Nov 26;12(22):8711-21</Citation><ArticleIdList><ArticleId IdType="pubmed">6095209</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell. 2010 Mar;22(3):623-39</Citation><ArticleIdList><ArticleId IdType="pubmed">20354195</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant J. 1999 Aug;19(4):387-98</Citation><ArticleIdList><ArticleId IdType="pubmed">10504561</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Mol Biol. 1997 Nov;35(4):443-58</Citation><ArticleIdList><ArticleId IdType="pubmed">9349268</ArticleId></ArticleIdList></Reference><Reference><Citation>New Phytol. 2011 Nov;192(3):626-39</Citation><ArticleIdList><ArticleId IdType="pubmed">21819406</ArticleId></ArticleIdList></Reference><Reference><Citation>Gene. 1999 Jan 21;226(2):181-8</Citation><ArticleIdList><ArticleId IdType="pubmed">9931484</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2006 Sep 15;313(5793):1596-604</Citation><ArticleIdList><ArticleId IdType="pubmed">16973872</ArticleId></ArticleIdList></Reference><Reference><Citation>Ann Bot. 2002 Dec;90(6):681-9</Citation><ArticleIdList><ArticleId IdType="pubmed">12451023</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 1982 Jul 9;217(4555):149-51</Citation><ArticleIdList><ArticleId IdType="pubmed">17770257</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 2001 Dec;127(4):1399-404</Citation><ArticleIdList><ArticleId IdType="pubmed">11743081</ArticleId></ArticleIdList></Reference><Reference><Citation>Am J Clin Nutr. 2004 May;79(5):727-47</Citation><ArticleIdList><ArticleId IdType="pubmed">15113710</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell Rep. 2006 May;25(5):403-9</Citation><ArticleIdList><ArticleId IdType="pubmed">16369767</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant J. 1995 Nov;8(5):659-71</Citation><ArticleIdList><ArticleId IdType="pubmed">8528278</ArticleId></ArticleIdList></Reference><Reference><Citation>J Exp Bot. 2005 Sep;56(419):2573-85</Citation><ArticleIdList><ArticleId IdType="pubmed">16087700</ArticleId></ArticleIdList></Reference><Reference><Citation>Oecologia. 2000 Apr;123(1):99-107</Citation><ArticleIdList><ArticleId IdType="pubmed">28308750</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant J. 1999 Jul;19(1):81-5</Citation><ArticleIdList><ArticleId IdType="pubmed">10417729</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Mol Biol. 1994 Mar;24(5):743-55</Citation><ArticleIdList><ArticleId IdType="pubmed">8193299</ArticleId></ArticleIdList></Reference><Reference><Citation>Biotechnol Lett. 2010 Sep;32(9):1325-32</Citation><ArticleIdList><ArticleId IdType="pubmed">20464449</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant J. 2002 Dec;32(5):701-12</Citation><ArticleIdList><ArticleId IdType="pubmed">12472686</ArticleId></ArticleIdList></Reference><Reference><Citation>Annu Rev Plant Biol. 2007;58:435-58</Citation><ArticleIdList><ArticleId IdType="pubmed">17280524</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2004 Sep 21;101(38):13951-6</Citation><ArticleIdList><ArticleId IdType="pubmed">15353603</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 2007 Nov;145(3):601-15</Citation><ArticleIdList><ArticleId IdType="pubmed">17885080</ArticleId></ArticleIdList></Reference><Reference><Citation>New Phytol. 2005 Jan;165(1):9-28</Citation><ArticleIdList><ArticleId IdType="pubmed">15720617</ArticleId></ArticleIdList></Reference><Reference><Citation>Ecology. 2007 Mar;88(3):729-39</Citation><ArticleIdList><ArticleId IdType="pubmed">17503600</ArticleId></ArticleIdList></Reference><Reference><Citation>Phytochemistry. 2006 Apr;67(7):684-95</Citation><ArticleIdList><ArticleId IdType="pubmed">16524606</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><noCountry><name sortKey="Gou, Jiqing" sort="Gou, Jiqing" uniqKey="Gou J" first="Jiqing" last="Gou">Jiqing Gou</name><name sortKey="Jia, Zhichun" sort="Jia, Zhichun" uniqKey="Jia Z" first="Zhichun" last="Jia">Zhichun Jia</name><name sortKey="Luo, Keming" sort="Luo, Keming" uniqKey="Luo K" first="Keming" last="Luo">Keming Luo</name><name sortKey="Song, Feng" sort="Song, Feng" uniqKey="Song F" first="Feng" last="Song">Feng Song</name><name sortKey="Sun, Yimin" sort="Sun, Yimin" uniqKey="Sun Y" first="Yimin" last="Sun">Yimin Sun</name><name sortKey="Xiao, Xunyan" sort="Xiao, Xunyan" uniqKey="Xiao X" first="Xunyan" last="Xiao">Xunyan Xiao</name><name sortKey="Yang, Li" sort="Yang, Li" uniqKey="Yang L" first="Li" last="Yang">Li Yang</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Huang, Yan" sort="Huang, Yan" uniqKey="Huang Y" first="Yan" last="Huang">Yan Huang</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PoplarV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002987 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 002987 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= PoplarV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:22363429
|texte= Molecular cloning and characterization of two genes encoding dihydroflavonol-4-reductase from Populus trichocarpa.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:22363429" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a PoplarV1
| This area was generated with Dilib version V0.6.37. |  |